RESUMO
Background: Histoplasmosis, caused by the dimorphic fungus Histoplasma capsulatum, represents an important public health problem, especially in urban environments where bats and humans cohabit indoors. Aims: To detect the presence of H. capsulatum indoors, using samples of bat droppings collected in roost sites inside houses. Methods:A Real-Time TaqMan PCR assay targeting the ITS1 region of the ribosomal NA of H. capsulatum was carried out. Results: Fifty-nine sampling points in the municipality of São Paulo were inspected, all of them located at inhabited places. H. capsulatum was isolated from nine samples. Conclusions: The rapid identification and monitoring of sites where the fungus is present may contribute to make a more reliable database of H. capsulatum distribution
Antecedentes: La histoplasmosis, causada por el hongo dimorfo Histoplasma capsulatum, representa un importante problema de salud pública, especialmente en los entornos urbanos donde los murciélagos y los humanos conviven en los espacios interiores. Objetivos: Detectar la presencia de H. capsulatum en interiores mediante muestras de excrementos de murciélagos recogidas en sitios de reposo de estos animales dentro de las casas. Métodos: Se llevó a cabo un ensayo de PCR TaqMan(R) en tiempo real dirigido a la región ITS1 del ADN ribosómico de H. capsulatum. Resultados: Se muestrearon 59 puntos en el municipio de São Paulo, todos ubicados en lugares habitados. H. capsulatum se aisló en nueve de estos lugares. Conclusiones: Una rápida identificación y control de los sitios donde se encuentra H. capsulatum contribuiría a la creación de una base de datos más sólida en lo referente a la distribución de este hongo
Assuntos
Animais , Quirópteros/microbiologia , DNA Fúngico/isolamento & purificação , Proteínas Fúngicas/genética , Histoplasma/genética , Habitação , Brasil , DNA Ribossômico , Fezes/microbiologia , Proteínas Fúngicas/isolamento & purificação , Histoplasma/isolamento & purificação , Histoplasmose/microbiologia , Reação em Cadeia da Polimerase em Tempo Real , Saúde da População UrbanaRESUMO
A total of 2,582 specimens of mosquitoes of 16 taxonomic categories grouped into 5 genera (Aedes, Culex, Mansonia, Toxorhynchites, and Wyeomyia) were collected in a central park of São Paulo City, Brazil. It is crucial to include such an area in official entomological surveillance programs since this park has all the epidemiological characteristics needed to maintain an enzootic cycle of arboviruses.
